Fate, behaviour and microbial response of diluted bitumen and conventional crude spills in a simulated warm freshwater environment.

Diluted bitumen (DB), one of the most transported unconventional crude oils in Canada's pipelines, raises public concerns due to its potential spillage into freshwater environments. This study aimed to compare the fate and behaviour of DB versus conventional crude (CC) in a simulated warm freshwater environment. An equivalent of 10 L of either DB or CC was spilled into 1200 L of North Saskatchewan River (NSR) water containing natural NSR sediment (2.4 kg) in a mesoscale spill tank and its fate and behaviour at air/water temperatures of 18 °C/24 °C were monitored for 56 days. Oil mass distribution analysis showed that 42.3 wt % of CC and 63.6 wt% of DB resided in the oil slicks at the end of 56-day tests, consisting mainly high molecular weight (HMW) compounds (i.e., resins and asphaltenes). The lost oil contained mainly low molecular weight (LMW) compounds (i.e., light saturates and some aromatics) into the atmosphere, water column, and sediment through collective weathering processes. Notably, weathered CC emulsified with water and remained floating until the end, while the weathered DB mat started to lose its buoyancy after 24 days under quiescent conditions and resurfaced once waves were applied. Analysis of the microbial communities of water pre- and post-spills revealed the replacement of indigenous microbial communities with hydrocarbon-degrading species. Exposure to CC reduced the microbial diversity by 12%, while exposure to DB increased the diversity by 10%. During the early stages of the spill (up to Day 21), most dominant species were positively correlated with the benzene, toluene, ethylbenzene, and xylenes (BTEX) content or polycyclic aromatic hydrocarbon (PAH) content of the water column, while the dominant species at the later stages (Days 21-56) of the spill were negatively correlated with BTEX or PAH content and positively correlated with the total organic carbon (TOC) content in waters.

Response of microbial communities in North Saskatchewan River to diluted bitumen and conventional crude under freeze-thaw-refreeze cycle.

Microorganisms are the first responder to oil spills and their response provides insight into the ecological effects of oils on aquatic ecosystems. Limited information is available about the impact of oil spills on freshwater ecosystems under seasonal river-ice regimes. This study aimed to investigate the microbial response of North Saskatchewan River water to diluted bitumen (DB) and conventional crude (CC) during the freeze-thaw-refreeze cycle. In two separate experiments, equivalent to 2 L of fresh DB and CC were spilled on the ice-covered river water within a mesoscale spill tank. The microbial response (changes in abundance and diversity) to oils under the freeze, thaw, and refreeze cycles were assessed for 10 days using 16S rRNA gene sequencing. The results showed that microbial communities exhibited different responses to the DB and CC oils. The effect of oils was more pronounced than that of the freeze or thaw cycles. The river microbial community rapidly responded to both spills, which coincided with a steady increase in the organic content of water throughout the freeze-thaw-refreeze cycle. Microbial diversity increased after the DB spill, but remain unchanged after the CC spill, regardless of the cycles. A higher number of new taxa emerged during the ice-covered period, while more microbial enrichment (increase in abundance) was observed during the thaw cycle. Flavobacterium (37 ± 5%) and Pseudomonas (36 ± 4%) remained the most predominant genera post-DB and CC spill, respectively. The results of this study suggest that ice coverage of 5 cm did not prevent the microbial communities from the effects of oils. Thus, a quick clean-up response to an oil spill on ice-covered water is equally critical to avoid the effects of oils on the underlying freshwater ecosystems.

Global phosphorus retention by river damming.

More than 70,000 large dams have been built worldwide. With growing water stress and demand for energy, this number will continue to increase in the foreseeable future. Damming greatly modifies the ecological functioning of river systems. In particular, dam reservoirs sequester nutrient elements and, hence, reduce downstream transfer of nutrients to floodplains, lakes, wetlands, and coastal marine environments. Here, we quantify the global impact of dams on the riverine fluxes and speciation of the limiting nutrient phosphorus (P), using a mechanistic modeling approach that accounts for the in-reservoir biogeochemical transformations of P. According to the model calculations, the mass of total P (TP) trapped in reservoirs nearly doubled between 1970 and 2000, reaching 42 Gmol y(-1), or 12% of the global river TP load in 2000. Because of the current surge in dam building, we project that by 2030, about 17% of the global river TP load will be sequestered in reservoir sediments. The largest projected increases in TP and reactive P (RP) retention by damming will take place in Asia and South America, especially in the Yangtze, Mekong, and Amazon drainage basins. Despite the large P retention capacity of reservoirs, the export of RP from watersheds will continue to grow unless additional measures are taken to curb anthropogenic P emissions.